Fig. 1

Single-cell transcriptomic atlas of the human amnion. A Workflow of single-cell RNA-sequencing analysis. B, C The t-distributed stochastic neighbor embedding (t-SNE) plot of 72,818 cells profiled with each cell color-coded to indicate different cell clusters (B) and cell types (C). 14 clusters and 6 major cell types were identified. FB, fibroblasts; EpC, epithelial cells; ImC, immunocytes; EpC_FB, epithelial_fibroblasts; ImC_EpC, immune_epithelial cells and ImC_FB, immune_fibroblasts. D, E Feature (D) and dot (E) plots showing the expression of established markers of epithelial cells, fibroblasts and immunocytes. Dot size encodes the percentage of cells expressing the gene, and color encodes the average level of gene expression per cell. F Co-staining of epithelial/fibroblast markers E-cadherin (green)/vimentin (red) or KRT14 (green)/N-cadherin (red). G Co-staining of epithelial/immunocyte markers E-cadherin (green)/CD45 (red) or KRT14 (green)/CD45 (red). H Co-staining of fibroblast/immunocyte markers vimentin (green)/CD45 (red) or N-cadherin (green)/CD45 (red). Nuclei were counterstained blue with DAPI. Scale bar, 20 μm