Table 4 Primers used for Real-time RT-PCR
Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
β-actin | GGAGATTACTGCCCTGGCTCCTA | GACTCATCGTACTCCTGCTTGCTG |
P16INK4A | AACACTTTCGGTCGTACCC | GTCCTCGCAGTTCGAATC |
Rb1 | CATGCTAGCAGCTGTTCTTTAC | AGTTTGCCTATATCAGCACAGT |
ALDOA | TGTTGTGGGCATTAAGGTAGA | GAGGGACGAGGGAGTATGC |
ENO1 | CAAGTCCTTCATCAAGAACTATCC | TGTCAGGTCATCTCCCACAAT |
HK1 | TCTAAACTCTGGGAAACAAAGGT | GAAGTCAATCAGGATGTTACGG |
HK2 | GCCGTAGTGGACAAGATAAGAGAG | GCCTCCATACCCATCTTGCTAC |
LDHA | TCCCTGAAGTCTCTGAACCCG | TCCTCCTTGATTCCATAGAGACC |
LDHB | GGTGGTGGACAGTGCCTATGA | GACTTCGTTCTCGATGCCGTA |
PFKM | GTGAAAGACCTGGTGGTTCAGA | CTCTGAGATCTTGCCTTCCTTC |
PK | GAAACAGCCAAAGGGGACTA | GGACTCCGTCAGAACTATCAAA |
PGAM1 | GAGCGACACTATGGGGGTCTAAC | TCTGCGTACCTGCGGTCCTT |
TPI1 | CACTCTGAACGCAGCCAAGC | CACCCAGGTAGCTCCTAAGTCC |