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Fig. 5 | Cell & Bioscience

Fig. 5

From: Single-cell genomics identifies distinct B1 cell developmental pathways and reveals aging-related changes in the B-cell receptor repertoire

Fig. 5

scBCR-seq reveals the clonal expansion of B1 cell with age. a The bar plot of the D50 metric quantifying the paired VH-VL amino acid sequences (see Methods). b The stacked bar plot shows the distribution of the top clonal proportions of both B1 and B2 cells of differentially aged mice. The colors represent different clonotype indices, clonotypes were ranked by the clonotype counts. c The stacked bar plot shows the distribution changes of the rare clonal proportions of both B1 and B2 cells of differentially aged mice. The colors represent different clonotype counts. d Variable germline gene segment pairing for B-cell receptor repertoires of B1 cells from differentially aged mice. Heatmaps show the percentage of B1 cell clones with a particular VH-VL pairing. Row and column histograms indicate marginal VH and VL frequencies, respectively. The top 20 enriched VH and VL genes were shown in each age. e The clonotype tracking bar plot shows the dynamic change of the specific B1 cell clones’ proportions with age. The BCR repertoire was analyzed by the R package immunarch [100] (see Methods)

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