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Fig. 4 | Cell & Bioscience

Fig. 4

From: Single-cell genomics identifies distinct B1 cell developmental pathways and reveals aging-related changes in the B-cell receptor repertoire

Fig. 4

Characterizing the gene signature of aged B1 cells. a The mature B1 cells (Bhlhe41+Fcer2aLy86+) of the initial UMAP projection (highlighted in orange), which was re-grouped into five clusters (B1-C1 to B1-C5) based on Harmony corrected latent space. b Bar plot showing the distributions of cells from the five B1 clusters for the six indicated groups of differentially aged mice. c Volcano plot showing the differentially expressed genes (DEGs) between B1-C5 and B1-C3 cells, DEGs (FDR < 0.01) were plotted. The aging-related genes were highlighted in red. d Violin plots comparing expression of cellular senescence related genes in different B1 clusters. The y axis indicates the log normalized expression level. e FACS plots showing expression of CD36 in CD19+CD43+CD23 B1 cells among the mice of indicated ages. Young adult (week 8, n = 3), elderly (month 14, n = 3) and elderly (month 20, n = 3). f The bar plot shows the statistics of the percentage of CD36+ cells in B1 cells in FACS analysis. The error bars indicate the s.e.m. *P < 0.5. n.s. not significant

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