Fig. 3

Dissecting the trajectory of neonate peritoneal B1 cell development. a The dot plot demonstrates marker genes expression in the C5-C9 clusters. b, c FACS plots validate that C5-C9 clusters are present in the neonate peritoneal cavity. Neonates (day 5–6, pool of 16 pups from 2 litters) were used for analysis. d FACS plots show the adoptive transfer results. Two weeks after injection of indicated cells directly into the peritoneal cavity of Rag2^−/− recipient mice (n = 3 for each donor cell type), peritoneal cells of recipient mice were isolated and analyzed by flow cytometry. Neonates (day 5–6, pool of 14 pups from 2 litters) were used as the donors. e The Palantir-derived UMAP plot shows another visualization of C1-C9 cells. f The diffusion pseudotime of C1-C9 cells was analyzed by Palantir (see Methods). The color key indicates the pseudotime from early (blue) to late (dark red). g UMAP plots showing the V(D)J rearrangement status of Igh and Igk chains in C1-C9 cells (see Methods). True means that the expression of rearranged V(D)J gene segments was captured by the B-cell receptor sequecning. Neonates (day 6, pool of 7 pups from 1 litter) were used for B cell receptor sequecning analysis. h Distribution of κ and λ light chain constant region gene expression in the Palantir-derived UMAP plot. The color key indicates the expression level. Data are representative of two (b and c) or there d independent experiments