Fig. 2

Lrp4 was expressed in the layer II of the piriform cortex. A Lrp4^+/Lac mice brain slice was co-stained with X-gal and anti-GFAP. GFAP positive cells were mainly distributed in the second superficial area of the piriform cortex, and X-gal staining positive cells were primarily located in layer IIa of the piriform cortex; nuclei were counterstained fast red. B Layer II of the piriform cortex. X-gal co-stained with GFAP⁺ cells (red arrow) and GFAP⁻ cells (white arrow; Lrp4^+/Lac mice, n = 5). C Percent of GFAP⁺ and GFAP⁻ cells in LacZ⁺ cell population in Layer IIa of the piriform cortex (Lrp4^+/Lac mice n = 3). D Representative images of neuron staining (anti-NeuN) of the two types of mice brain. E The thickness of the piriform cortex in layers I, II, and III did not change in Lrp4^ECD/ECD mice. F The neuronal density was not different in the three layers (Mice per type, n = 7. Scale bar = 50 μm. Values were means ± SEM. **P < 0.01)