Fig. 4

RND1 suppressed the growth of GBM in vivo. A The establishment efficacy of stable cell line with luciferase was assessed by western blot analysis. B Mouse survival was shown by Kaplan–Meier curves. U87-Luc groups, n = 14; U87-Luc-RND1 groups, n = 14. P value was calculated using the log-rank test. P = 0.0011. C RND1 suppressed the growth of GBM in the intracranial xenograft model. Luciferase fluorescence of mouse xenografts were detected by an IVIS at the 21st day after tumor injection. Luciferase activities were quantified. D The weight of tumors were analysed (Luc/Luc-RND1 groups—PBS groups). E Representative HE staining images of orthotopic tumor sections confirmed the tumor suppression in Luc-RND1 groups. F Protein levels of p53, SLC7A11, and RND1 in mouse tumor were also evaluated by western blot and quantified. IHC staining and western blot revealed that RND1 regulated the expression of p53 and SLC7A11 in vivo. Luc, Luciferase. *P < 0.05; **P < 0.01; ***P < 0.001