Fig. 3

RND1 regulated the expression of SLC7A11 and p53 in GBM. A Influence of RND1 expression on the protein levels of SLC7A11, GPX4, ACSL4 and FTL in U87. B Representative images of IHC staining showed that RND1 expression inversely correlates with SLC7A11 in the same location of GBM tissues. Scale bars, 50 μm at high magnification and 200 μm at low magnification. C Microarray analysis of 53 GBM tissues showed the inverse correlations of IHC data for high or low RND1 expression relative to level of SLC7A11. D mRNA level of SLC7A11 when RND1 was overexpressed or knocked down in U87. E, F Influence of RND1 expression on the protein levels of p53, NRF2, ATF3 and ATF4 in U87. G Western blot analysis showed that RND1 expression positively correlates with p53 protein level in clinical GBM samples. H Representatives images of IHC staining showed that RND1 expression positively correlates with p53 in the same location of GBM tissues. Scale bars, 50 μm at high magnification and 200 μm at low magnification. I Microarray analysis of 53 GBM tissues showed the positive correlations of IHC data for high or low RND1 expression relative to level of p53. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance