Fig. 6From: VHL Ser65 mutations enhance HIF2α signaling and promote epithelial-mesenchymal transition of renal cancer cellsVHL-S65P gene signature is associated with human kidney cancer progression. A Heatmap showing the expression profiles of genes differentially altered by VHL-S65P and directly bound by HIF2α. B ChIP-qPCR confirmation of HIF2α chromatin binding at the representative genes of VHL mutant signature in the cells expressing WT or mutant VHL (n = 3 replicates). C Analysis of mRNA expression of the VHL mutant signature genes in 786-O cells expressing VHL WT or mutants using quantitative RT-PCR (n = 3). D–E VHL-S65P signature is significantly upregulated in human renal cancers compared to normal kidney samples. F–I The expression of VHL-S65 signature positively correlate with EMT score (F–H) and hypoxia signaling score (I)Back to article page