Fig. 6
From: TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling
TRPM8 inhibitor mitigates liver fibrosis in CCl4-treated mice. A H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in liver sections of CCl4-treated mice (n = 5 per group). Image J was used to quantify positively stained areas. Scale bars, 100 μm. B Serum levels of ALT and AST were measured in mice (n = 5 per group). C Expressions of α-SMA and COL1A1 were detected by immunoblotting (n = 3 per group). D Hepatic mRNAs of fibrogenic genes were measured by qRT-PCR assays in mice treated with M8-B or DMSO after CCl4 induction (n = 5 per group). The results are expressed as mean ± SD. *P < 0.05, **P < 0.01