Fig. 5

TRPM8 regulates the expression of S100A9 and HNF4α. A Volcano plot and heatmap presentation of significantly up- and downregulated genes in liver specimens obtained from CCl₄-treated TRPM8^−/− mice compared to WT (n = 3 per group). B Serum S100A9 expressions in liver cirrhosis patients and healthy donors. C, E Histology of S100A9 and HNF4α IHC staining in liver sections obtained from CCl₄- or BDL-treated WT and TRPM8^−/− mice (n = 5 per group). Scale bars, 50 μm. D, F Expressions of S100A9 and HNF4α were detected by immunoblotting using liver specimens obtained from CCl₄- or BDL-treated WT and TRPM8^−/− mice (n = 3 per group). G Immunoblotting assays of TRPM8, S100A9, and HNF4α in primary hepatocytes from WT and TRPM8^−/− mice. H Immunoblotting assays of TRPM8, S100A9, and HNF4α in L02 cells transfected with TRPM8 siRNA after 48 h. I, J Immunoblotting assays of S100A9 and HNF4α in L02 cells transfected with S100A9 siRNA or plasmid after 48 h. The results are expressed as mean ± SD. **P < 0.01