Fig. 4

TRPM8 deficiency attenuates bile canalicular abnormalities, dysregulation of bile transporters, ductular reaction and liver inflammation in fibrotic liver. A Electron microscopy images show bile canaliculi (arrows) in liver sections obtained from CCl₄-treated WT and TRPM8^−/− mice. B, C Hepatic mRNAs of bile acid transport genes were measured by qRT-PCR in liver specimens obtained from CCl₄- or BDL-treated WT and TRPM8^−/− mice (n = 5 per group). D, F Histology of CK19 and F4/80 IHC staining in liver sections obtained from CCl₄- or BDL-treated WT and TRPM8^−/− mice. Positive staining areas were quantified by Image J software. Scale bars, 100 μm. E, G Hepatic mRNAs of IL-6, IL-1β, TNFα, and MCP-1 were measured by qRT-PCR in liver specimens obtained from CCl₄- or BDL-treated WT and TRPM8^−/− mice (n = 5 per group). The results are expressed as mean ± SD. *P < 0.05, **P < 0.01