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Fig. 2 | Cell & Bioscience

Fig. 2

From: TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling

Fig. 2

Liver fibrosis is attenuated in TRPM8−/− mice after CCl4 treatment. A Representative histology of H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in the liver of WT and TRPM8−/− mice induced by CCl4 (n = 5 per group). Positive staining areas were quantified by by Image J software. Scale bars, 100 μm. B Liver function was assessed by measuring the serum levels of ALT and AST in mice (n = 5 per group). C Immunoblotting analyses of α-SMA and COL1A1 expression in the mouse liver (n = 3 per group). D Hepatic mRNA levels of fibrogenic genes were measured by qRT-PCR (n = 5 per group). The results are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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