Fig. 7

FYN mediates the pro-metastatic role of PA2G4. A Cells were transfected with siRNA targeting FYN for 72 h. Western blot analysis of the knocking down efficacy by siRNA. GAPDH as loading control. B Representative images (B1) and quantification (B2) of the migrated and invaded cells in the indicated groups. Scale bar, black bar, 100 μm. C Cells with PA2G4 stably knocked down were transfected with pcDNA3.1-FYN to transient overexpressing FYN. Western blot analysis of the indicated proteins. GAPDH as loading control. D Cells were treated as in C. Representative images (D1) and quantification (D2) of the migrated and invaded cells in the indicated groups. Scale bar, black bar, 100 μm. Data was shown as Mean ± SD. Two-tailed Student t test for (B2 and D2). *p < 0.05, **p < 0.01. pc3.1: pcDNA3.1