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Fig. 5 | Cell & Bioscience

Fig. 5

From: PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner

Fig. 5

YTHDF2 is an endogenous binding patterner of PA2G4. A qRT-PCR of the relative FYN mRNA expression after treating with actinomycin D for the indicated time in PA2G4 knocked down cells. Data normalized to HPRT1 and are shown as percentage of the relative level in 0 h. B RIP assay for the binding of FYN mRNA with PA2G4 in the indicated cells. Data was shown as percentage of FYN mRNA in the anti-PA2G4 precipitates against 10% input. C HEK-293T cells were transduced with pcDNA3.1-PA2G4 plasmids to overexpress PA2G4, and then subjected to anti-PA2G4 immunoprecipitation. Sliver staining of the protein enriched by anti-PA2G4 antibody in HEK-293T cells. Black arrow pointed to PA2G4 bands. D The peptide spectrum of YTHDF2 by mass spectrum assay. E, F The binding between PA2G4 and YTHDF2 were detected by endogenous immunoprecipitation in HEK-293T (E) and in HCC cells (F). G Representative confocal images of the co-localization of YTHDF2 and PA2G4. Red, YTHDF2; green, PA2G4; Blue, DAPI. Scale bar, white bar, 10  µM. H RIP assay for the binding of FYN mRNA with YTHDF2 in the indicated cells. Data was shown as percentage of FYN mRNA in the anti-YTHDF2 precipitates against 10% input. Data was shown as Mean ± SD. Two-tailed Student t test for (A, B and H). **p < 0.01. IP: immunoprecipitation; IB: immunoblotting

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