Fig. 4

FYN is a downstream effector of PA2G4 in HCC. A Heatmap of differentially expressed genes between HLF/shPA2G4 and its control cells. Triplicates were used for RNA-Seq in each group. B, C KEGG (B) and GO (C) analyses for the upregulated and downregulated genes after knocking down PA2G4 in HLF cells. Red color was used to highlight the cancer related or cell mobility related terms. D HLF cells with PA2G4 knocked down and Huh7 cells with PA2G4 overexpressed were applied to found out the positive regulated downstream effectors of PA2G4 (log2(FC) ≥ 1 and p value < 0.05). Venn diagram for overlapping the downregulated genes in HLF/shPA2G4 cells (green circle) and the upregulated genes in Huh7/PA2G4 cells (red circle), compared with their respective control cells (D1). qRT-PCR of FYN expression in the indicated cells (D2). Data normalized to GAPDH and are shown as the fold change to their respective control cells. E Western blot analyses of FYN after manipulating PA2G4 level in Huh7 and HLF cells. GAPDH as loading control. Data was shown as Mean ± SD. Two-tailed Student t test for (D2); Overrepresentation analysis test for (B and C). *p < 0.05, **p < 0.01