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Fig. 2 | Cell & Bioscience

Fig. 2

From: PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner

Fig. 2

Upregulating PA2G4 induces partial EMT of HCC cell in vitro and promotes lung metastasis in vivo. A Western blot analyses of endogenous PA2G4 protein level in primary HCC cell lines. B Western blot analyses for PA2G4 expression in HCC cell lines with elevated bone metastatic abilities. BM: bone metastasis. C PA2G4 overexpression efficacy in the indicated cell lines were evaluated by western blot (C1) and qRT-PCR (C2). Data normalized to GAPDH and are shown as the fold change to their respective control cells. D Representative images of migration and invasion assay in the PA2G4 overexpressing HCC cells (upper panel). Scale bar: black bar, 100 μm. Quantification of cells migrated and invaded (lower panel). E Phalloidin staining (red) for F-actin in PA2G4 overexpressing HCC cells. DAPI (blue) for nuclei. Magnification, 400-fold. F Western blot analysis of the indicated epithelial to mesenchymal transition related protein markers in PA2G4 overexpressing cells. G Lung metastases formation after tail-vein injection of Huh7/PA2G4 cells. H&E staining for lung metastatic foci (G1); Lung metastatic incidence (G2) and metastatic numbers (G3) in mice bearing Huh7/vec or Huh7/PA2G4 cells were calculated. Scale bar: red bar, 200 μm in the overview images; 50 μm in the magnified images. GAPDH as loading control in (A, B, C1 and F). Data was shown as Mean ± SD. Two-tailed Student t test for (C2, D and G3). **p < 0.01. vec: vector

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