Fig. 6

CCR2A is involved in the proliferation and migration of HeLa cells. A Wild-type (WT) and CCR2A knockout (KO) HeLa cells were seeded in 96-well plates with medium supplemented with 10% fetal bovine serum (FBS, upper graph) or medium supplemented with 1% FBS and 100 ng/ml monocyte chemoattractant protein-1 (MCP-1, lower graph). Cells were subjected to cell counting kit 8 (CCK-8) assay every 24 h for 4 days. **p > 0.01 compared with WT cells. B Reverse transcriptase–polymerase chain reaction (RT-PCR) for MCP-1 gene with total RNAs isolated from WT or CCR2A KO HeLa cells. β-actin was used as a control. C Transwell migration assay. Cells were loaded in the upper chamber at a density of 1 × 10⁴ cells/well, and 100 ng/ml MCP-1 was added to the lower chamber. After 24 h, the transmembrane was fixed, the remaining cells in the upper wells were removed, and the migrated cells were stained. The cells that migrated to the lower surface of the transmembrane were counted in four different areas. ##p > 0.01 compared with WT, non-treated (NT), **p > 0.01 compared with WT treated with MCP-1. All experiments were conducted at least three independent times