Fig. 3
From: Analysis of CCR2 splice variant expression patterns and functional properties
MCP-1–dependent interactions between Gβ1 and G protein–coupled receptor kinases (GRKs) were mediated by both splice variants. A HEK293 cells expressing CCR2-LgBiT and Gβ1-SmBiT were treated with monocyte chemoattractant protein-1 (MCP-1). Molecular interaction was assessed by measuring luciferase activity. B Cells expressing CCR2B-LgBiT and SmBiT-β-arrestin 1 were pretreated with different doses of Cmpd101, a GRK2/3 inhibitor, and then stimulated with MCP-1 in the presence of luciferase substrate. C Cells expressing CCR2B or CCR2A with different Gβ1 and GRK NanoBiT constructs were treated with MCP-1, and luciferase activity was detected with a luminometer. D MCP-1-dependent interaction between CCR2B and GRK2. Cells co-expressing the NanoBiT forms of CCR2A or CCR2B together with GRK2 or GRK5 were treated with MCP-1, and luciferase activity was measured