Fig. 6
ATF6 activators-induced changes in sIPSC pharmacology. A sIPSCs mediated by wild-type α1β2γ2 GABAA receptors. B sIPSCs mediated by cells transfected with α1, β2 and γ2(R177G) subunits. (C) sIPSCs mediated by cells transfected with α1, β2 and γ2(R82Q) subunits. D sIPSCs mediated by cells transfected with α1(D219N), β2 and γ2 subunits. E Group bar plots showing the effect of DZP treatment on sIPSC peak amplitude for the indicated GABAA receptors. Normalized DZP effect (%Ipeak) = Ipeak (DZP) / Ipeak (control) * 100. The control Ipeak values were obtained from Fig. 5E. F Group bar plots showing the effect of DZP enhancement on sIPSC decay times for the indicated GABAA receptors. Normalized DZP effect (%Idecay) = decay time constant (DZP) / decay time constant (control) * 100. The control decay time constant values were obtained from Fig. 5F. G Group bar plots showing Zn2+ inhibition of sIPSC peak amplitude for the indicated GABAA receptors. Zn2+ inhibition (%Ipeak) = (Ipeak (control)—Ipeak (Zn2+)) / Ipeak (control) * 100. The control Ipeak values were obtained from Fig. 5E. For all panels, cells were incubated with DMSO (black, left), AA263 (5 μM, 24 h, blue, middle), or AA147 (5 μM, 24 h, red, right). Event frequency was ~ 0.2 Hz in control and drug treated cells. Asterisks represent p values for the post-hoc comparisons of a two-way ANOVA with and without AFT6 activators exposure, where * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001. Number signs represent the p values for a one-way ANOVA without ATF6 activators exposure, where ##p < 0.01, ###p < 0.005 and ####p < 0.0001. ANOVAs are always compared to α1β2γ2 GABAA receptors