Fig. 5
ATF6 activators-induced changes to sIPSC peak current and decay times. A sIPSCs mediated by wild-type α1β2γ2 GABAA receptors. B sIPSCs mediated by cells transfected with α1, β2 and γ2(R177G) subunits. C sIPSCs mediated by cells transfected with α1, β2 and γ2(R82Q) subunits. D sIPSCs mediated by cells transfected with α1(D219D), β2 and γ2 subunits. E Group bar plots showing changes in sIPSC peak amplitude for the indicated GABAA receptors. F Group bar plots showing changes in sIPSC decay times for the indicated GABAA receptors. For all panels, cells were incubated with DMSO (black, left), AA263 (5 μM, 24 h, blue, middle), or AA147 (5 μM, 24 h, red, right). Event frequency was ~ 0.2 Hz in control and drug treated cells. G Image of co-culture showing a cluster of primary neurons, axonal extensions and a nearby recorded HEK293 cell that is labelled with CD4 antibody beads. Asterisks represent p values for the post-hoc comparisons of a two-way ANOVA with and without AFT6 activators exposure, where * p < 0.05, ** p < 0.01, *** p < 0.005. Number signs represent the p values for a one-way ANOVA without ATF6 activators exposure, where # p < 0.05 and #### p < 0.0001. ANOVAs are always compared to α1β2γ2 GABAA receptors