Fig. 7

miR-let-7b promotes hADSC senescence, which is reversed by LYPLAL1-AS1. A qRT-PCR of miR-let-7b in hADSCs transfected with miR-let-7b mimic, NC, or miR-let-7b inhibitor. B SA-β-Gal staining in hADSCs transfected with miR-let-7b mimic, NC, or miR-let-7b inhibitor. C qRT-PCR of P16, P21, and LMNB1 in hADSCs transfected with miR-let-7b mimic, NC, or miR-let-7b inhibitor. D Western blot of P16, P21, and LMNB1 in hADSCs transfected with miR-let-7b mimic, NC, or miR-let-7b inhibitor. E qRT-PCR of miR-let-7b, P16, P21, and LMNB1 in hADSCs transfected with miR-let-7b mimic and (or) LYPLAL1-AS1 expression vector. F Western blot of P16, P21, and LMNB1 in hADSCs transfected with miR-let-7b mimic and (or) LYPLAL1-AS1 expression vectors. qPCR data were normalized to GAPDH, n = 3. Data are shown as mean ± SD from three independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; scale bars: 200 µm