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Fig. 6 | Cell & Bioscience

Fig. 6

From: LncRNA LYPLAL1-AS1 rejuvenates human adipose-derived mesenchymal stem cell senescence via transcriptional MIRLET7B inactivation

Fig. 6

LYPLAL1-AS1 negatively regulates MIRLET7B. A Genome visualization of the LYPLAL1-AS1 binding site within the MIRLET7B promoter region detected by ChIRP-seq. B The relative firefly to Renilla luciferase activity (upper panel, n = 3) and LYPLAL1-AS1 expression (lower panel) of 293 T cells co-transfected with MIRLET7B promoter reporter vector (GPL4-let7b promoter), LYPLAL1-AS1 expression vector (pcDNA3.1-LYPLAL1-AS1), and Renilla luciferase vector (pRL-TK). C The relative firefly to Renilla luciferase activity (upper panel, n = 3) and LYPLAL1-AS1 expression (lower panel) of 293 T cells co-transfected with MIRLET7B promoter reporter vector (GPL4-let7b promoter), LYPLAL1-AS1 expression vector (pcDNA3.1-LYPLAL1-AS1), Renilla luciferase vector (pRL-TK), and LYPLAL1-AS1 siRNAs or control siRNAs. D qRT-PCR of miR-let-7b in LYPLAL1-AS1-silenced or -overexpressed hADSCs. E qRT-PCR of miR-let-7b in hADSCs during replicative senescence. F qRT-PCR of miR-let-7b in H2O2-treated hADSCs. qPCR data were normalized to GAPDH, n = 3. Data are shown as mean ± SD from three independent experiments; **p < 0.01, ***p < 0.001, ****p < 0.0001

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