Fig. 3
LYPLAL1-AS1 knockdown accelerates hADSC senescence. A qRT-PCR of siRNA-mediated knockdown of LYPLAL1-AS1. B SA-β-Gal staining of control and LYPLAL1-AS1-silenced P10 hADSCs. C qRT-PCR of P16, P21, and LMNB1 in control and LYPLAL1-AS1-silenced P10 hADSCs. D Western blot of P16, P21, and LMNB1 in control and LYPLAL1-AS1-silenced P10 hADSCs. E qRT-PCR of P16, P21, and LMNB1 in control and LYPLAL1-AS1-silenced H2O2-treated hADSCs. F Western blot of P16, P21, and LMNB1 in control and LYPLAL1-AS1-silenced H2O2-treated hADSCs. G SA-β-Gal staining in control and LYPLAL1-AS1-silenced H2O2-treated hADSCs. qPCR data were normalized to GAPDH, n = 3. Data are shown as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; scale bars: 200 µm