Fig. 2
From: MLL-AF4 and a murinized pSer-variant thereof are turning on the nucleolar stress pathway
Overexpression of mCherry-tagged versions of MA4 and MA4m, and their intracellular localization. A Series of pictures taken with a fluorescence microscope showing the intracellular localization of UBF, a transcription factor necessary for RNA Pol I transcription inside the nucleolus. B Distribution of fusion genes and RNA Pol II in mock cells, as well as cells overexpressing the MA4::mCh and MA4m::mCh fusion proteins. Intracellular localization of both fusion proteins was observed in the nucleus, but also in nucleous (stronger in MA4 than in MA4m cells). C ChIP Experiments of genomic DNA performed with α-mCherry antibody and an α-RNA Pol II antibody. The two fusion proteins were unable to precipitate the investigated rRNA gene promoters, but the experiment with the α-RNA Pol II antibody revealed RNA Pol II binding to the investigated rRNA promoter when MA4::mCh was overexpressed