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Fig. 6 | Cell & Bioscience

Fig. 6

From: p52 signaling promotes cellular senescence

Fig. 6

Senescent cells upregulate p52 Chromatin enrichment. a-c ChIP-seq analysis of WI-38 cells at D0 and D7 following prolonged culture. a Heatmap of differential binding of endogenous p52 to gene promoters. b Histograms demonstrating average peak intensities of ChIP-Seq data from WI-38 cells at Day 0 (lower) and Day 7 (upper). Pie charts demonstrate the distribution of p52 Chip-Seq peaks annotated to specific genomics features on indicated day. Primary binding motifs also shown. c UCSC genome browser view of p52 binding peaks at candidate gene regulatory elements on the indicated day. d ChIP-qPCR analysis of p52 chromatin enrichment at indicated gene on day 0 or 7 following culture of WI-38 cells. Data show mean enrichment at specific gene relative to input and IgG control, ± SEM of two independent experiments. e qPCR analysis of indicated genes in WI-38 cells expressing sh-RNA targeting NFKB2 (right) or a scrambled sequence (SCR, left). Data show mean expression relative to GAPDH normalized to day 0, ± SEM of three independent experiments. *P < 0.05. **P < 0.01 (two-tailed t test)

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