Fig. 1

Cellular senescence is associated with increased nuclear p52. a Immunoblot (IB) using lysate from mouse embryonic fibroblasts (MEFs) at passage 2 (P2) and 5 (P5). IB performed with anti-p52, anti-p50, and anti-Gapdh. b qPCR analysis of indicated mRNA in MEFs at P2 and P5. Data show mean value of triplicate biological samples relative to Gapdh, ± SEM normalized to P2. c IB using nuclear extract from MEFs probed with indicated antibodies. d Quantification of β-gal staining of MEFs on Day 0 (D0) and 7 (D7). Representative images (left). Data show mean value of at least 200 cells per sample of three biological repeats, ± SEM. e IB probed with anti-p52 using lysate from MEFs at D0, D5, and D7. f Quantification of β-gal staining of WI-38 cells at P19 and P46. Representative images (left). g IB using lysate from WI-38 cells probed with the indicated antibodies. h Quantification of β-gal staining in WI-38 cells at D0 and D7. i and j IB using lysate from WI-38 cells at Day 0 and D7 probed with the indicated antibodies. Scale bar, 100 μm. ** P < 0.01 (two-tailed t test). Blots are representative of at least two biologically independent experiments. Analysis of fold-change normalized to control lane shown below IB where indicated