Fig. 7

Tumor slice culture response to KU-60019 treatment and radiation. A Schematic of orthotopic transplantation of GFP positive KP6 and KPP4 cells to immune competent C57BL/6 mice. The picture shows GFP positive tumors in mouse lungs after 8 weeks. The tumor bearing mouse lungs were cut by vibratome and cultured in 10% FCS/DMEM in 24 well plates. Culture slices were treated with DMS0 or ATM inhibitor and irradiated with 0 Gy or 8 Gy. B Tissue slices (n = 2–4) of transplanted KP6 (black) and KPP4 (blue) were pre-treated with DMSO (continuous line) or 3 µM Ku60019 (dashed line) Treated tissue slices were observed and pictures of same tumor sites were taken for 8 days. The fluorescent signal of the tumor area was measured, and background area was subtracted. On the Graphs the GFP signal over time with different treatment conditions is shown. Below each graph are typical pictures of measured tumor sites illuminated with standardized 488 nm led light source and same camera settings. C Tissue slices (n = 2–4) of transplanted KP6 (black) and KPP4 (blue) were pre-treated with DMSO (continuous line) or 3 µM Ku60019 (dashed line) and irradiated with 8 Gy. Treated tissue slices were observed and pictures of same tumor sites were taken for 8 days. The fluorescent signal of the tumor area was measured, and background area was subtracted. On the Graphs the GFP signal over time with different treatment conditions is shown. Below each graph are typical pictures of measured tumor sites illuminated with standardized 488 nm led light source and same camera settings. Also see Additional file 1: Fig. S7