Skip to main content
Fig. 2 | Cell & Bioscience

Fig. 2

From: PTEN mutant non-small cell lung cancer require ATM to suppress pro-apoptotic signalling and evade radiotherapy

Fig. 2

Generating and characterizing murine PTEN deficient tumor cell lines. A Representative haematoxylin and eosin (H&E) staining of tumor bearing animals 12 weeks post intratracheal infection. On the left KP (KRasG12D:Tp53mut) on the right KPP (KRasG12D:Tp53mut:Ptenmut). Boxes indicate upper highlighted tumor areas. B Representative haematoxylin and eosin (H&E) and immunohistochemical DAB staining (PTEN, p-ERK1/2 and p-S6) of tumor bearing animals 12 weeks post intratracheal infection. On the upper part KP (KRasG12D:Tp53mut) on the lower part KPP (KRasG12D:Tp53mut:Ptenmut). C Quantification of % tumor area (normalized to total lung area) in KP (black) and KPP (blue) animals. n = 3. D Kaplan–Meier survival curves comparing KP (black; n = 5) and KPP (blue, n = 5) animals upon AAV intratracheal infection. E Immunoblot of endogenous (phospho-)AKT of two representative generated cell lines from different mice. KP5 and KP6 (KRasG12D:Tp53mu), KPP4 and KPP8 (KRasG12D:Tp53mut:Ptenmut). Actin as loading control. n = 3. F Colony formation assay KP5 (gray), KP6 (black), KPP4 (blue) and KPP8 (light blue). SF 2: Surviving fraction at 2 Gy. D25: Dose in Gy with 25% survival. Error bars: Standard deviation. n = 3. G Immunoblot against PTEN/Pten of KP6 and lentivirally transduced, either GFP or human PTEN cDNA overexpressing KPP4 cells after Puromycin selection. Actin as loading control. n = 3. H Colony formation assay KPP4 (blue) and PTEN reconstituted KPP4 clones (C5, C7, C15 and C18; gray to black; Additional file 1: Fig. S3D) after clonogenic isolation. SF 2: Surviving fraction at 2 Gy. D25: Dose in Gy with 25% survival. Error bars: Standard deviation. n = 3. Also see Additional file 1: Fig. S2

Back to article page