Fig. 1
From: 3D and organoid culture in research: physiology, hereditary genetic diseases and cancer
Generation of pancreatic ductal organoids from mouse and human pancreases and possible direct applications. Pancreatic tissue extract is digested and cultured to generate tissue-specific organoids. Organotypic cultures allow the creation of progression models in vivo, as well as the analysis and subsequent validation of proteins and genes involved in human tumor progression by phenotypic or histologic analysis