Fig. 4
PA1 is required for normal transcription in Sertoli cells. a Schematic diagram depicting transcriptome analysis of Sertoli cells. Briefly, Total RNA of wild type and Pa1-knockout Sertoli cells from 20-day-old mice testes were collected for the RNA-seq. b Heatmaps depicting significantly upregulated and downregulated genes in Pa1F/F (upper) and Amh-pa1−/− Sertoli cells (lower). The genes with |log2 Fold change|≥ 1 and q < 0.05 were determined to generate the heatmap. c Volcano plot showing DEGs in Pa1F/F and Amh-pa1−/− Sertoli cells. The significant changed downregulated genes associated with Sertoli functions were labeled with gene name. d GO-Biological process analysis of 1045 downregulated genes in Amh-pa1−/− Sertoli cells. Biological process of cell adhesion, phagocytosis, positive regulation of gene expression, cell surface receptor signaling pathway, and cortical actin cytoskeleton organization were labeled with asterisks. e RT-qPCR of DEGs identified in the volcano plot including Elmo1, Gdnf, Tgfb3, Sfrp1, Hgf, Met and Cx43 relative to Gapdh in Pa1F/F and Amh-pa1−/− Sertoli cells. (n = 4). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001