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Fig. 3 | Cell & Bioscience

Fig. 3

From: PA1 participates in the maintenance of blood–testis barrier integrity via cooperation with JUN in the Sertoli cells of mice

Fig. 3

The integrity of BTB is disrupted in Amh-pa1−/− mice. a The integrity of the BTB was observed to be destroyed in 8-week-old Amh-pa1−/− testes. Immunofluorescence using FITC-labeled streptavidin (green) was conducted on seminiferous tubules of Pa1F/F (upper) and Amh-pa1−/− testes (lower) which had been injected with biotin beneath the albuginea. Nuclei were stained with DAPI (blue). Dashed boxes showed localization of the enlarged images. Asterisks indicated biotin penetrated seminiferous tubules. b Transmission electron microscope images of adult Pa1F/F and Amh-pa1−/− mice testes showing the cleft junctions between Sertoli cells at the basal compartment of seminiferous tubules. Red dashed boxes showed localization of the enlarged images shown in the bottom row. Red dashed lines in the enlarged images indicate the cell–cell junctions. c BTB components are shown to be disorganized in 8-week-old Amh-pa1−/− mice testis. Immunofluorescence analysis using anti-Claudin11, anti-Occludin, anti-ZO-1, and anti-β-catenin was conducted in the testis of Pa1F/F mice (left) and Amh-pa1−/− mice (right). Nuclei were stained with DAPI (blue). Arrowheads indicate the aberrant patches or diminished signals of the BTB components. Dashed curve indicated basal membrane of seminiferous tubules. d Immunoblot analysis of BTB components, including ZO-1, Occludin, Claudin11, and β-Catenin, in the testes of adult wild-type mice and Amh-pa1−/− mice. e Cell junction cannot be formed between Amh-pa1−/− Sertoli cells. Immunofluorescence using anti-ZO-1 (green) was conducted on Sertoli cells of Pa1F/F (upper) and Amh-pa1−/− testes (lower). Nuclei were stained with DAPI (blue). Dashed boxes showed localization of the enlarged images

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