Fig. 8
From: BRD4 inhibitor GNE987 exerts anti-cancer effects by targeting super-enhancers in neuroblastoma
FAM163A is a potential oncogene of NB. A IGV plots showing ChIP-seq profiles of FAM163A in NB gene locus. The ChIP-seq gene tracks represent the H3K27ac signal in normal neural crest cells (green) (GSE90683), NB cell lines (red) (GSE90683) and clinical samples from NB (orange) (GSE90805). B FAM163A mRNA expression level in a broad range of tumours (generated from CCLE: https://portals.broadinstitute.org/ccle). C Relative mRNA expression of FAM163A in normal nerve cells and NB cells based on data available from the GEO database. D Overall survival (OS) in patients with NB with high or low levels of FAM163A plotted using Kaplan–Meier method (generated from R2 Genomics Analysis and Visualization Platform: r2.amc.nl). p < 0.001. E SK-N-BE (2) cells were transfected with sh-NC, sh-FAM163A #A, or sh-FAM163A #B and positive cells were screened using puromycin for 6 days. The mRNA expression of FAM163A was assessed using qRT-PCR. F Microscopic images of the SK-N-BE (2) cells and SK-N-BE (2) cells transfected with sh-NC, sh-FAM163A #A, or sh-FAM163A #B. G–H Representative images and statistical results of a clone formation assay showing the colony formation ability of the SK-N-BE (2) cells stably transfected with sh-NC, sh-FAM163A #A, or sh-FAM163A #B. *p < 0.05, **p < 0.01. I Proliferation rate of the SK-N-BE (2) cells transfected with sh-NC, sh-FAM163A #A, or sh-FAM163A #B. ****p < 0.0001