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Fig. 7 | Cell & Bioscience

Fig. 7

From: BRD4 inhibitor GNE987 exerts anti-cancer effects by targeting super-enhancers in neuroblastoma

Fig. 7

Comprehensive analysis of RNA-seq and ChIP-seq identified novel candidate oncogenic transcripts. Volcano plot of gene expression differences between the GNE987-treated and DMSO-treated SK-N-BE (2) cells. The blue and red dots highlight all the statistically significant transcripts with downregulated/upregulated expression (log2FoldChange < − 1.0 or > 1, adjusted p < 0.05). B Enrichment analysis results of differentially expressed genes by using GSEA Pathway Database to investigate the function of GNE987 in gene regulation. C Enhancers were ranked by increasing H3K27ac signal in the GNE 987 treated and nontreated SK-N-BE (2) cells. The number of SEs is shown for each group. D Venn diagram showing the 297 overlapping genes between the RNA-seq decreased genes and SE-related genes calculated from ChIP-seq results. The relative expression levels of the representative overlapping genes are depicted in the heatmap, of which the peak score and fold change are shown in the table. E RT-qPCR analysis of genes related to SE from SK-N-BE (2) treated with DMSO, 5 nM or 10 nM for 24 h. ****p < 0.0001

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