Fig. 5

TSLP increased mitophagy-related protein expression in THP-1 cells via histone modification. The protein signal intensities of A PINK1, B phospho-Parkin (p-parkin), and C LC3 after treatment with different concentrations (0, 2, 10, 40 ng/mL) of TSLP for 8 h was determined by western blotting. The standard deviations of the optical density data were calculated from three independent experiments, and one representative result of the set of three is shown. *p < 0.05 and **p < 0.01 for the comparison with and without TSLP treatment. D Confocal laser microscopy showed that TSLP (10 ng/mL) treatment for 24 h significantly increased PINK1 signals and promoted PINK1 colocalization with mitochondria. Pretreatment with the histone acetyltransferase inhibitor AA and the histone methyltransferase inhibitor MTA reduced TSLP-induced PINK1 signals in mitochondria. E Confocal laser microscopy showed that TSLP (10 ng/mL) treatment for 24 h significantly increased LC3 signals and promoted LC3 colocalization with mitochondria. Pretreatment with AA and MTA reduced TSLP-induced LC3 signals in mitochondria, as determined by confocal laser microscopy. Confocal laser microscopy showed that TSLP (10 ng/mL) treatment for 24 h significantly increased F phosphor-parkin (p-Parkin) and G phospho-ubiquitin (p-Ubiquitin) signals and promoted mitophagy-related protein colocalization with mitochondria