Fig. 4

TSLP promoted respiratory chain complex II SDHA expression via epigenetic regulation. TSLP-induced SHDA expression was inhibited by A the histone acetyltransferase inhibitor anacardic acid (AA) and B the histone methyltransferase inhibitor methylthioadenosine (MTA). Cells were pretreated with AA or MTA for 1 h prior and then stimulated with TSLP for 2 h. ChIP analysis revealed that TSLP enhanced C H3 acetylation and D H4 acetylation in the SHDA promoter. TSLP also significantly enhanced H3K4 and H3K36 trimethylation (E and G) but did not enhance H3K9 trimethylation (F) in the SHDA promoter. *p < 0.05, **p < 0.01 and ***p < 0.001 between THP-1 cells with and without TSLP treatment