Fig. 2

P62 negatively regulates SLC7A11 expression in p53-mutant GBM cells but positively regulates SLC7A11 expression in p53-wild-type GBM cells. A U251, U118, U87 and A172 cells were transfected with pcDNA3.1 or HA-P62 plasmids, followed by erastin treatment. The protein expression of SLC7A11, NRF2, P53, Keap1 and P62 were detected by western blot analysis. The quantification of western blots were also presented. B U251 and U87 cells were transfected with pcDNA3.1 or HA-P62 plasmids, followed by DMSO or erastin treatment. The mRNA expression of P62 and SLC7A11 were measured by qPCR. mRNA expression was normalized to GAPDH using the 2^−ΔΔCt method. C CRISPR/CAS9 system was performed to knockout p62 expression in U251 cells. SLC7A11 and p62 expression were detected by western blot analysis