Fig. 6

PI3K/MAPK signaling is involved in ZC3H4-mediated anoikis resistance in response to SiO₂. A Representative western blot showing that macrophage CM increased the levels of PI3K/MAPK-related proteins in HPF-a cells. B Densitometry analyses of three separate experiments indicated that macrophage CM increased the levels of phosphorylated Akt, JNK, Erk, and P38 in HPF-a cells (n = 3); *P < 0.05 and ***P < 0.001 compared with the corresponding protein level detected at 0 h. C Representative western blot showing that CM-induced ZC3H4 and NTRK2 expression was attenuated by the pretreatment of HPF-a cells with a MAPK or PI3K/Akt inhibitor. D Densitometry analyses of ZC3H4 expression from 4 separate experiments and TrkB expression from 3 separate experiments. **P < 0.01 and ***P < 0.001 compared with the corresponding control group; ###P < 0.001 compared with the corresponding CM group