Fig. 5
SiO2-induced pulmonary inflammation activates ERS, but not autophagy, in HPF-a cells. A Representative western blot showing that macrophage CM upregulated ERS-related protein expression in HPF-a cells. B Densitometry analyses of five separate experiments (n = 5); *P < 0.05, **P < 0.01, and ***P < 0.001 compared with 0 h. C HPF-a cells were treated with tunicamycin (an ERS agonist), and ZC3H4 and NTRK2 expression was upregulated in response to tunicamycin treatment. D Densitometry analyses of five separate experiments (n = 5); *P < 0.05 compared with the control. E HPF-a cells were treated with salubrinal (an ERS inhibitor) for 6 h, and western blot analysis showed that ZC3H4 and NTRK2 levels were downregulated in response to salubrinal treatment. F Densitometry analyses of five separate experiments (n = 5); *P < 0.05 compared with the control