Fig. 4

ZC3H4 is involved in anoikis resistance-mediated fibroblast activation in response to SiO₂. A Representative western blot showing that macrophage CM upregulated ZC3H4 expression in HPF-a cells. B Densitometry analyses of five separate experiments suggested that macrophage CM upregulated ZC3H4 expression in HPF-a cells (n = 5); *P < 0.05 and **P < 0.001 compared with 0 h. C Representative western blot analysis suggesting that poly-HEMA attenuated CM-induced increases in ZC3H4 expression in HPF-a cells. D Densitometry analyses of five separate experiments (n = 5); *P < 0.05 compared with the control group. E Representative western blot showing the effects of ZC3H4-NIC transfection on ZC3H4 and NTRK2 expression in HPF-a cells. F Densitometry analyses of three separate experiments suggested that ZC3H4 NIC transfection affected CM-induced increases in ZC3H4 and TrkB expression in HPF-a cells (n = 3); *P < 0.05 and **P < 0.001 compared with the corresponding control group. G Spatial transcriptomics showing the coexpression of Zc3h4 and Vimentin