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Fig. 3 | Cell & Bioscience

Fig. 3

From: A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation

Fig. 3

SiO2-CM promotes fibrosis and anoikis resistance in HPF-a cells growing in ECM. A Schematic diagram of fibroblasts transplanted to ECM after decellularization. B Cell counting showed the effect of SiO2-CM on MLG cell proliferation cultured in ECM (n = 5). *P < 0.05 compared with the control group. C In the 3D migration experiment, the migration of fibroblasts increased after 24 h of exposure to SiO2-CM. Scale bar = 275 μm. D Statistical results of the number of migrating fibroblasts in the 3D migration experiment and the maximum migration distance after 24 h of exposure to SiO2-CM (n = 5). *P < 0.05 compared with the control group and **P < 0.01 compared with the control group. Immunofluorescence staining showing the expression of the fibroblast markers Vimentin (E) and α-SMA (F) and the anoikis protein NTRK2 to detect the effect of SiO2-CM on MLG cells. Scale bar = 200 μm

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