Fig. 2
Anoikis resistance-mediated fibroblast activation in response to SiO2. A HPF-a cells were treated with Con-CM and SiO2-CM for 24 h and double stained with Annexin V and PI. The sum of the counts in Q2-1, Q2-2 and Q2-4 was defined as the number of apoptotic cells. B Statistical analysis of three independent experiments of HPF-a cell apoptosis using FCM. C The CCK-8 assay detected the viability of HPF-a cells after treatment with Con-CM and SiO2-CM for 24 h (n = 5). ***P < 0.001 compared with the control group. D HPF-a cells were cultured in wells pretreated with poly-HEMA for 48 h and collected for counting (n = 5). ***P < 0.001 compared with the control group. E HPF-a cells were suspended for 48 h, and cell viability was detected using the CCK-8 assay (n = 5). ***P < 0.001 compared with the control group. F HPF-a cells were suspended for 48 h, and the expression level of TrkB was detected (n = 5). ***P < 0.001 compared with the control group. G HPF-a cells were suspended for 48 h before they were treated with Con-CM and SiO2-CM for 24 h and double stained with Annexin V and PI. The sum of the counts in Q2-1, Q2-2 and Q2-4 was defined as the number of apoptotic cells. H Statistical analysis of three independent experiments of HPF-a cell apoptosis using FCM; ***P < 0.001 compared with the attached group. I Statistical analysis of five independent experiments of HPF-a cell viability using the CCK-8 assay (n = 5); ***P < 0.001 compared with the attached group. J Spatial transcriptomics showed the coexpression of apoptosis markers (Bim1) and Vimentin. K Spatial transcriptomics showing the coexpression of proliferation markers (Cdk1) and Vimentin