Fig. 6From: YTHDF1 promotes breast cancer progression by facilitating FOXM1 translation in an m6A-dependent mannerYTHDF1 regulates FOXM1 expression in an m6A-dependent manner. A The analysis of qPCR for FOXM1 expression in RIP assay (anti-Flag) in MDB-MA-231 and MCF-7 cells. B FOXM1 protein expression detected by western blot in MDB-MA-231 and MCF-7 cells transfected with YTHDF1-WT or YTHDF1-MUT plasmids. C Schematic image of FOXM1-WT plasmids, and FOXM1-MUT plasmids containing m6A motif mutations in the CDS region. D Protein expression of HA tagged FOXM1 in MDA-MB-231 and MCF-7 cells co-transfected with empty vector, YTHDF1-WT or YTHDF1-MUT, and FOXM1-WT or FOXM1-MUT plasmids. All data were presented as means ± SD of at least three independent repetitions. Values are significant at *P < 0.05, **P < 0.01, ***P < 0.001 as indicatedBack to article page