Fig. 5

FOXM1 is a direct target of YTHDF1. Normalized expression of target genes detected by RT-qPCR after A RIP, B m6A-IP or C YTHDF1-CLIP assay. D Correlation between FOXM1 protein expression and YHTDF1 mRNA expression in TCGA-BRCA dataset. E FOXM1 protein expression in breast cancer tissue and normal tissue from The Human Protein Atlas. F The protein and expression of YTHDF1 and FOXM1 in MDA-MB-231 and MCF-7 cells transduced with sh-NC, sh-YTHDF1#1 or sh-YTHDF1#2. G The correlation between YTHDF1 and FOXM1 in protein levels by immunohistochemical analysis in 9 samples. H The mRNA expression of YTHDF1 and FOXM1 in MDA-MB-231 and MCF-7 cells transduced with sh-NC, sh-YTHDF1#1 or sh-YTHDF1#2. I Polysome profiling assay in MCF-7 cells transduced with sh-NC or sh-YTHDF1#2 (Left). FOXM1 and 18 s rRNA expression in different polysome fractions (Right). All data were presented as means ± SD of at least three independent repetitions. Values are significant at *P < 0.05, **P < 0.01, ***P < 0.001 as indicated