Fig. 5
From: Regulation of paternal 5mC oxidation and H3K9me2 asymmetry by ERK1/2 in mouse zygotes
ERK1/2 Inhibition promotes the accumulation of 5mC and 5hmC in paternal pronuclei. A Representative immunofluorescence images of 5mC (green), 5hmC (red) in control and ERK1/2-inhibited (50 μM GDC-0994) zygotes at different stages. The pronuclear (PN) stages are indicated. Quantification is represented as the mean of 5mC and 5hmC signal intensity in parental pronuclei after background subtraction (B, C, mat. and D, E, pat.). F, G Quantification of 5mC (left) and 5hmC (right) staining as a ratio between the pronuclear signals (pat/mat). Number of zygotes analyzed for each stage: Early PN3, 13; Late PN3, 11; PN5, 23 for control and Early PN3, 14; Late PN3, 10; PN5, 20 for GDC-0994. Each data point represents a zygote. P values are indicated. Error bars indicate SD. ♀, maternal pronucleus; ♂, paternal pronucleus. PB, polar body. Scale bar, 20 µm