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Fig. 3 | Cell & Bioscience

Fig. 3

From: Regulation of paternal 5mC oxidation and H3K9me2 asymmetry by ERK1/2 in mouse zygotes

Fig. 3

ERK1/2 impedes the deposition of paternal H3K9me2. A H3K9me2 deposition in ERK1/2-inhibited (100 μM GDC-0994) zygotes at different developmental stages. The pronuclear (PN) stages are indicated. Quantification of the H3K9me2 signal intensity in parental pronuclei (B, C) and the paternal to maternal signal ratio of H3K9me2 (D) in ERK1/2-inhibited zygotes. Number of zygotes analysed for each stage: Early PN3, n = 10; Late PN3, n = 11; PN5, n = 14 for control and Early PN3, n = 9; Late PN3, n = 19; PN5, n = 14 for GDC-0994-treatment. Each data point represents an independent zygote. E Representative images show equal distribution of H3K9me2 between paternal and maternal genomes in ERK1/2-inhibited (100 μM GDC-0994) 2-cell embryos compared to the controls. F The expression of H19 and Igf2 in ERK1/2-inhibited blastocysts. P values are indicated. Error bars indicate SD. ♀, maternal pronucleus; ♂, paternal pronucleus. PB, polar body. Scale bar, 20 µm

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