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Fig. 9 | Cell & Bioscience

Fig. 9

From: Siah-1-interacting protein regulates mutated huntingtin protein aggregation in Huntington’s disease models

Fig. 9

Mutations in the N-terminal domain of SIP decrease its ability to protect mHTT against aggregation. a HEK293T cells were co-transfected with plasmids (control pEntry and e1HTT-72Q-RFP; wt SIP and e1HTT-72Q-RFP; SIP K21W and e1HTT-72Q-RFP; SIP T30R_S33E and e1HTT-72Q-RFP) and imaged under a fluorescent microscope (left part of panel a). Images in the right part of the panel a were taken in transmitted light. The size and number of mHTT aggregates (e1HTT-72Q) are presented in panels b and c, respectively. b Red color signals of e1HTT-72Q were measured as the number of pixels per aggregate. Panel b shows the average aggregate size measured for each of the analyzed images. The results are expressed as mean ± SEM. Scale bar = 1 µM. **p < 0.01; ***p < 0.001. ns, not significant. The results were obtained from three independent HEK293T culture preparations

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Cell & Bioscience

ISSN: 2045-3701

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