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Fig. 4 | Cell & Bioscience

Fig. 4

From: Siah-1-interacting protein regulates mutated huntingtin protein aggregation in Huntington’s disease models

Fig. 4

SIP increases autophagy in cells that express N-terminal mutant HTT but not in the presence of N-terminal wt HTT. a HEK293T cells were co-transfected with plasmids (control pEntry and e1HTT-25Q-RFP; wt SIP and e1HTT-25Q-RFP; control pEntry and e1HTT-72Q-RFP; wt SIP and e1HTT-72Q-RFP), pretreated with rapamycin and chloroquine, and imaged for autophagy using the Cyto-ID Autophagy detection kit. Signals from autophagosomes were detected in green. Signals that corresponded to the expression of proteins that were encoded by e1HTT-25Q-RFP and e1HTT-72Q-RFP were detected in red and yellow, respectively. Aggregates of e1HTT-72Q were imaged in yellow (white arrows) because of their high fluorescence intensity, which was detected in the FITC channel. Scale bar = 1 µM. b Area of autophagosomes in double-transfected HEK293T cells using the above plasmids. The area of autophagosomes was measured as the number of pixels per e1HTT expressed cell

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