Fig. 2

Presence of SIP dimers in YAC128 MSN culture. a, b Immunofluorescence of SIP (magenta), MAP2 (green), and nuclei (Hoechst 33,342; blue) in YAC128 and wildtype MSNs, respectively. Scale bar = 10 µm. c, d Proximity ligation assay (PLA) signal (red) of SIP dimers and nuclei (Hoechst 33,342; blue) in YAC128 MSNs. e Negative control for PLA without primary antibodies. Scale bar = 5 µm. Fluorescence signals were detected by three-dimensional analysis that was performed from the top of MSN cultures. The results of Z-axis analyses are presented either above or to the right of the main panels. The analysis was performed below nuclei (A), through the middle of nuclei (B), and above nuclei (C). White arrows indicate SIP protein localization in the cytoplasm and nucleus a, b and SIP dimers at cytoplasmic compartments (c, d)