Table 2 Summary of antibodies used in this paper
Antibody | Species | Vendor | Cat.NO | WB dilution | IF dilution | ||
|---|---|---|---|---|---|---|---|
HP1 alpha | Rb | Abcam | ab109028 | 1/3000 | 1/250 | ||
H3K9me3 | Rb | Abcam | ab176916 | N/A | 1/200 | ||
H3K27me3 | Rb | Abcam | ab6002 | N/A | 1/200 | ||
SOX10 | Rb | Abcam | ab155279 | 1/1500 | 1/100 | ||
Nestin | Rb | Beyotime | AF2215 | N/A | 1/50 | ||
LMNB2 | Rb | Beyotime | AF0219 | 1/1000–1/5000 | 1/200 | ||
Lamin B1 | Rb | Beyotime | AF1408 | 1/1500 | N/A | ||
Lamin B1 | Rb | Proteintech | 12987-1-AP | 1/2000 | 1/50 | ||
Lamin A/C | Rb | Proteintech | 10298-1-AP | 1/1000 | 1/100 | ||
Nanog | Ms | Santa Cruz | sc-374001 | N/A | 1/50 | ||
Oct-3/4 | Ms | Santa Cruz | sc-5279 | N/A | 1/50 | ||
SSEA-4 | Ms | Santa Cruz | sc-21704 | N/A | 1/50 | ||
TRA-1–60 | Ms | Santa Cruz | sc-21705 | N/A | 1/50 | ||
Progerin | Ms | Sigma | SAB4200272 | 1/3000 | 1/200 | ||
γH2A.X | Ms | Millipore | 05-636 | N/A | 1/100 | ||
53BP1 | Rb | Santa Cruz | sc-22760 | N/A | 1/50 | ||